期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 54, 期 392, 页码 2589-2591出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erg282
关键词
Key words; Gene expression; okadaic acid; potato stolons; StCDPK1; sucrose
StCDPK1 encodes a calcium-dependent protein kinase (CDPK) from Solanum tuberosum, which is transiently induced upon tuberization in swelling stolons. In situ hybridization determined that StCDPK1 mRNA is localized in the apical dome of tuberizing stolon tips, close to the region where sucrose was reported to accumulate. The expression of StCDPK1, and other tuber-specific genes was enhanced when in vitro-cultured potato plants were transferred to high sucrose or high sorbitol containing media. Glucose, fructose or a mixture of both showed no effect on CDPK expression. Okadaic acid blocked sucrose-inducible gene expression, suggesting that phosphatases from the PP1/PP2A family could also participate in the regulation of StCDPK1 and other tuberization-related genes.
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