期刊
CELL BIOLOGY INTERNATIONAL
卷 33, 期 7, 页码 749-754出版社
WILEY
DOI: 10.1016/j.cellbi.2009.04.010
关键词
Silicon dioxide; PARP; gamma-H2AX; RQ-PCR; Immunofluorescence
类别
资金
- National Natural Science Foundation of China [30872090]
- Program of Beijing Municipal Commission of Education [KM200810025021]
Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells treated with concentrations of silicon dioxide up to 400 mu g/ml was determined by real-time fluorogenetic quantitative PCR (RQ-PCR) and immunofluorescence assay, respectively. MTT assay was used to determine cell viability. The results showed that viability at 400 mg/ml silica was significantly decreased but not at lower concentrations. The protein content of gamma-H2AX in silica-treated group was significantly higher than the controls. The PARP mRNA and protein levels were significantly reduced with a dose response manner from the lowest silicon dioxide level. Our findings suggested that silicon dioxide increased the expression of gamma-H2AX and inhibited the expression of PARP mRNA and protein in Hela cells. (c) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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