期刊
INTERNATIONAL JOURNAL OF CANCER
卷 107, 期 2, 页码 177-182出版社
WILEY-LISS
DOI: 10.1002/ijc.11387
关键词
estrogen; tamoxifen; breast cancer; magnetic resonance spectroscopy; glucose; lactate
类别
资金
- NATIONAL CANCER INSTITUTE [R01CA042238] Funding Source: NIH RePORTER
- NCI NIH HHS [CA 42238] Funding Source: Medline
Estrogen plays a key role in the development and progression of breast cancer; hence, antiestrogens, such as tamoxifen, have a marked impact on the treatment and outcome of breast cancer patients. Estrogen-induced growth requires continuous replenishment of energy, predominantly generated by glycolysis. Previous work from this laboratory demonstrated estrogen induction and tamoxifen inhibition of glycolysis in MCF7 human breast cancer cells in vitro (Furman et at., J Steroid Biochem Mol Biol 1992;43:189-95). We present here studies of estrogen vs. tamoxifen regulation of glycolysis in orthotopic MCF7 human breast cancer xenografts in vivo. In addition we investigated mediation of this metabolic regulation through glucose transporter 1, in the same cells, in vitro, as well as in 2 other hormone-responsive human breast cancer cells. Tumor response and glycolysis were monitored noninvasively by means of magnetic resonance imaging and C-13 spectroscopy, respectively. During estrogen-stimulated 3 tumor growth (from approximate to0.5 to approximate to1.3 cm(3) in 10 days), the rate of glucose metabolism through glycolysis in vivo was high at 40 +/- 4 mumole/g/min. However, treatment for 10 days with tamoxifen induced growth arrest and a concomitant decrease of 2-fold in the rate of glycolysis. In congruence, glucose transporter 1 expression was stimulated by estrogen, reaching after 72 hr a 2- to 3-fold higher level of expression relative to that in tamoxifen-treated cells. Thus, estrogen-induced changes in glycolysis appeared to be mediated via its regulation of glucose transporter 1 expression. The in vivo monitoring of glycolysis may serve as a tool to expose hormonal regulation of glucose transporter 1 expression in breast cancer tumors, as well as to assess response to hormonal therapy. (C) 2003 Wiley-Liss, Inc.
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