期刊
CELL BIOLOGY INTERNATIONAL
卷 32, 期 10, 页码 1256-1264出版社
WILEY
DOI: 10.1016/j.cellbi.2008.07.010
关键词
Mesenchymal stem cells; Human heme oxygenase-1; Gene therapy; Myocardial cell injury
类别
资金
- Chinese National Nature Science Foundation [30700314]
- Program for Innovative Research Team of Northeast Agricultural University [CXZ008-1]
The aim has been to determine whether the supernatants of mesenchymal stem cells (MSCs) transfected with adenovirus carrying human heme oxygenase-1 (hHO-1) gene protect cardiomyocytes from ischemic injury. We have found that hHO-1 infected MSCs (hHO-1-MSCs) increased expression of hHO-1 protein. Apoptosis of cultured hHO-1-MSCs exposed to hypoxia was suppressed. Several cytokines, including HGF, bFGF, TGF-beta, VEGF and IL-1 beta, were produced by hHO-1-MSCs, some being significantly enhanced under hypoxia stimulation. Meanwhile, those cytokines reduced caspase-3 level and activity in cultured adult rat ventricular cardiomyocytes (ARVCs) exposed to hypoxia. Supernatants obtained from hHO-1-MSCs improved left ventricular function, limited myocardial infarct size, increased microvessel density, and inhibited apoptosis of cardiomyocytes in rat myocardial infarction. It can be concluded hHO-1-modified MSCs prevent myocardial cell injury via secretion of paracrine-acting mediators. (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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