期刊
CELL BIOLOGY AND TOXICOLOGY
卷 29, 期 3, 页码 189-197出版社
SPRINGER
DOI: 10.1007/s10565-013-9246-1
关键词
Apoptosis; Flow cytometry; Haemocyte; Harmful algal bloom; Okadaic acid
资金
- Conselleria de Innovacion e Industria, INCITE (Xunta de Galicia)
- Conselleria de Innovacion e Industria, Xunta de Galicia
- [07MMA013103PR]
Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms (HAB), belongs to the Diarrheic Shellfish Poisoning toxins that cause gastrointestinal symptoms in humans after consumption. In the present work, Ruditapes decussatus haemocytes were selected to evaluate the effect of OA on cell viability, enzymatic status and immune capacity through the measure by flow cytometry of apoptosis-cell death, non-specific esterase activity and phagocytosis. In order to compare different exposure conditions, two experiments were developed: in vitro exposure to OA and HAB simulation by feeding clams with the OA producer, Prorocentrum lima. Apoptosis was not OA dose-dependent and cell death increased in both assays. Phagocytosis of latex beads and esterase activity decreased in haemocytes incubated with OA. In contrast, esterases increased during the feeding with P. lima. Our results showed that OA and the simulated HAB caused damages on haemocyte functions and viability.
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