期刊
CELL BIOCHEMISTRY AND FUNCTION
卷 28, 期 7, 页码 620-622出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/cbf.1692
关键词
5 ' nucleotidase; concanavalin-A; glycoprotein; affinity chromatography; snake venom
资金
- Indian Council of Medical Research (ICMR), New Delhi, India
Pharmacologically active 5' nucleotidase is a ubiquitously distributed enzyme in snake venoms. In this study the effect of concanavalin A (Con-A) on different snake venoms 5' nucleotidase activity is tested in order to know the protein nature which will ultimately help in purification of the enzyme with high yield. Con-A inhibited Naja naja, Naja kauthia, Naja melanoleuca, Naja naja sputatrix, Agistrodon halys blomhoffii, Bothrops asper and Oxyranus scutellas venom 5' nucleotidase activity at different concentrations. This indicates the presence of glycopart in the protein, thus glycoprotein in nature. Vipera russellii, Vipera plaestenae, Agistrodon contratrix, Bitis orientis, Echis carinatus and Trimeresures malabaricus was not inhibited by Con-A, indicating absence of glycopart in the protein. This study for the first time shows existence of 5' nucleotidase in multimeric forms. Copyright (C) 2010 John Wiley & Sons, Ltd.
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