期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 375, 期 1, 页码 133-138出版社
SPRINGER-VERLAG BERLIN
DOI: 10.1007/s00216-002-1648-5
关键词
Se-methionine; yeast; Brazil nuts; hydrolysis; HPLC-ICP-MS
资金
- NIEHS NIH HHS [ES04908] Funding Source: Medline
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P42ES004908] Funding Source: NIH RePORTER
In this work, the use of methanesulfonic acid for protein hydrolysis is proposed for evaluation of Se-methionine in yeast, Brazil nuts, and possibly other selenium-rich biological samples. The hydrolysis was carried out by heating the sample with 4 mol L-1 acid at reflux for 8 h. Two chromatographic techniques (size-exclusion and ion-pairing) coupled with ICP-MS detection were used to compare the release of Se-methionine from proteins by enzymatic (proteinase K, protease XIV) and acid hydrolyses. A more efficient liberation of Se-methionine was observed by acid hydrolysis. For quantification, the sample extracts were introduced onto a C8 Alltima column, and the separation was achieved with a mobile phase containing 5 mmol L-1 hexanesulfonic acid in citrate buffer (pH 4.5)/methanol (95:5). The results obtained by standard addition showed 816+/-17 mug g(-1) and 36.2+/-1.5 mug g(-1) of selenium in the form of Se-methionine in yeast and nuts, respectively (65% and 75% of total selenium).
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