期刊
BIOTECHNOLOGY LETTERS
卷 25, 期 22, 页码 1941-1944出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/B:BILE.0000003990.27624.04
关键词
genomic DNA; Magnaporthe grisea; microwave treatment; PCR; RAPD
Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and for a quick screening of M. grisea transformants.
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