期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 420, 期 1, 页码 114-120出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.abb.2003.09.013
关键词
zinc; ascorbic acid; sodium-dependent vitamin C transporter; osteoblastic differentiation; MC3T3-E1 cells; northern blotting
Zinc is an essential trace element that increases osteoblast numbers and bone formation. However, the mechanisms involved in the Zn-induced differentiation of osteoblasts are poorly understood. We examined the roles of L-ascorbic acid (AA) and its transporter, sodium-dependent vitamin C transporter (SVCT) 2, in the Zn-induced expression of osteoblastic differentiation markers. Zinc time- and dose-dependently induced SVCT2 mRNA expression in the absence or presence of AA. Western blotting and kinetic assays showed that Zn increased functional SVCT2 protein levels and AA transport. In the presence of AA, 50 muM Zn enhanced mRNA expression of the osteoblastic differentiation markers alkaline phosphatase, alpha(1) (I) procollagen, osteopontin (OPN), and osteocalcin (OCN) by 3.9-, 3.8-, 3.3-, and 3.5-fold, respectively; in the absence of AA, the Zn-induced increase was 2.8-, 2.5-, 1.3-, and 1.1-fold, respectively. These findings suggest that AA and SVCT2 mediate Zn-induced OPN and OCN expression and partly regulate Zn-induced osteoblastic differentiation. (C) 2003 Elsevier Inc. All rights reserved.
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