期刊
BIOTECHNOLOGY LETTERS
卷 25, 期 1, 页码 51-54出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1021730131633
关键词
chiral alcohols; Lactobacillus brevis; NADH oxidase; oxidation
A new NADH oxidase, useful for the regeneration of NAD+, was isolated and characterized from Lactobacillus brevis. In crude extracts the activity was from 10-15 U mg(-1). After purification by four chromatographic steps, an activity of 116 U mg(-1) was obtained with 14% yield. Highest activity was from pH 5.5-7 and at 40degreesC. The enzyme requires dithiothreitol to prevent oxidative deactivation. The K-m value for NADH was 24 muM.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据