期刊
JOURNAL OF CELL SCIENCE
卷 116, 期 1, 页码 29-38出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.00182
关键词
gap junctions; dye transfer; Xenopus connexins; Cx30; Cx31; Cx38; Cx41; Cx43; Cx43.4
类别
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM037751, R37GM018974, R01GM018974] Funding Source: NIH RePORTER
- NIGMS NIH HHS [GM18974, GM37751] Funding Source: Medline
To explore the role of gap junctional intercellular communication (GJIC) during Xenopus embryogenesis, we utilized the host-transfer and antisense techniques to specifically deplete Cx38, the only known maternally expressed connexin. Cx38-depleted embryos developed normally but displayed robust GJIC between blastomeres at 32-128 cell stages, suggesting the existence of other maternal connexins. Analysis of embryonic cDNA revealed maternal expression of two novel connexins, Cx31 and Cx43.4, and a third, Cx43, that had been previously identified as a product of zygotic transcription. Thus, the early Xenopus embryo contains at least four maternal connexins. Unlike Cx38, expression of Cx31, Cx43 and Cx43.4 continue zygotically. Of these, Cx43.4 is the most abundant, accumulating significantly in neural structures including the brain, the eyes and the spinal cord.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据