期刊
MOLECULAR BIOLOGY OF THE CELL
卷 14, 期 1, 页码 129-141出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E02-08-0483
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- NCRR NIH HHS [RR-00592, P41 RR000592] Funding Source: Medline
- NIGMS NIH HHS [R01 GM059992, 5R01 GM362-11, GM-59992] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR000592] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM059992] Funding Source: NIH RePORTER
Nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae are formed through specific interactions between Vac8p on the vacuole membrane and Nvj1p in the nuclear envelope. Herein, we report that NV junctions in yeast promote piecemeal microautophagy of the nucleus (PMN). During PMN, teardrop-like blebs are pinched from the nucleus, released into the vacuole lumen, and degraded by soluble hydrolases. PMN occurs in rapidly dividing cells but is induced to higher levels by carbon and nitrogen starvation and is under the control of the Tor kinase nutrient-sensing pathway. Confocal and biochemical assays demonstrate that Nvj1p is degraded in a PMN-dependent manner. PMN occurs normally in apg7-Delta cells and is, therefore, not dependent on macroautophagy. Transmission electron microscopy reveals that portions of the granular nucleolus are often sequestered into PMN structures. These results introduce a novel mode of selective microautophagy that targets nonessential components of the yeast nucleus for degradation and recycling in the vacuole.
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