期刊
CELL AND TISSUE RESEARCH
卷 340, 期 1, 页码 61-69出版社
SPRINGER
DOI: 10.1007/s00441-010-0931-6
关键词
RECK; MMP-2; MMP-3; MMP-9; Palate development; ECM remodeling; Mouse
类别
资金
- PRP-USP
- CAPES
- FAPESP [01/10707-7, 08/53003-9]
- CNPq [350084/03-3, 475721/2003-9, 505350/04-1]
- FINEP [01.04.0469.00]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [01/10707-7] Funding Source: FAPESP
We have evaluated RECK (reversion-inducing-cysteine-rich protein with Kazal motifs), MMP-2 (matrix metalloproteinase-2), MMP-3, and MMP-9 involvement during palate development in mice by using various techniques. Immunohistochemical features revealed the distribution of RECK, MMP-2, and MMP-3 in the mesenchymal tissue and in the midline epithelial seam at embryonic day 13 (E13), MMPs-2, -3, and -9 being particularly expressed at E14 and E14.5. In contrast, RECK was weakly immunostained at these times. Involvement of MMPs was validated by measuring not only their protein expression, but also their activity (zymograms). In situ hybridization signal (ISH) for RECK transcript was distributed in mesenchymal and epithelial regions within palatal shelves at all periods evaluated. Importantly, the results from ISH analysis were in accord with those obtained by real-time polymerase chain reaction. The expression of RECK was found to be temporally regulated, which suggested possible roles in palatal ontogeny. Taken together, our results clearly show that remodeling of the extracellular matrix is finely modulated during secondary palate development and occurs in a sequential manner.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据