期刊
POULTRY SCIENCE
卷 82, 期 1, 页码 54-61出版社
POULTRY SCIENCE ASSOC INC
DOI: 10.1093/ps/82.1.54
关键词
sterol response element binding protein; transcription factor; localization; expression; chicken
Sterol regulatory element binding protein-1 and -2 (SREBP-1 and -2) are key transcription factors involved in-the biosynthesis of cholesterol and fatty acids. The SREBP have mainly been studied in rodents in which lipogenesis is regulated in both liver and adipose tissue. There is, however, a paucity of information on birds, in which lipogenesis occurs essentially in the liver as in humans. As a prelude to the investigation of the role of SREBP in lipid metabolism regulation in chicken, we sequenced the cDNA, encoding the mature nuclear form of chicken SREBP-2 protein, mapped SREBP-1 and -2 genes and studied their tissue expressions. The predicted chicken SREBP-2 amino acid sequence shows a 77 to 79% identity with human, mouse, and hamster homologues, with a nearly perfect conservation in all the important functional motifs, basic, helix-loop-helix, and leucine zipper (bHLH-Zip) region as well as cleavage sites. As in the human genome, SREBP-1 and SREBP-2 chicken genes are located on two separate chromosomes, respectively microchromosome 14 and macrochromosome 1. Tissue expression data show that SREBP-1 arid SREBP-2 are expressed in a wide variety of tissues in chicken. However, unlike SREBP-2, SREBP-1 is expressed preferentially in the liver and uropygial gland, suggesting an important role of SREBP-1. in the regulation of lipogenesis in avian species.
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