期刊
CELL
卷 174, 期 6, 页码 1586-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2018.07.009
关键词
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资金
- NWO Gravitation Program (NWO)
- KWF grant [HUBR2014-7006]
- KWF Queen Wilhelmina Award [NKI 2013-6122]
- ERC AdG SENSIT
Cancer immunotherapies have shown substantial clinical activity for a subset of patients with epithelial cancers. Still, technological platforms to study cancer T-cell interactions for individual patients and understand determinants of responsiveness are presently lacking. Here, we establish and validate a platform to induce and analyze tumor-specific T cell responses to epithelial cancers in a personalized manner. We demonstrate that co-cultures of autologous tumor organoids and peripheral blood lymphocytes can be used to enrich tumor-reactive T cells from peripheral blood of patients with mismatch repair-deficient colorectal cancer and non-small-cell lung cancer. Furthermore, we demonstrate that these T cells can be used to assess the efficiency of killing of matched tumor organoids. This platform provides an unbiased strategy for the isolation of tumor-reactive T cells and provides a means by which to assess the sensitivity of tumor cells to T cell-mediated attack at the level of the individual patient.
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