期刊
CELL
卷 174, 期 6, 页码 1373-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2018.08.039
关键词
-
资金
- Damon Runyon Cancer Research Foundation [DRG-2292-17, DRG-2017-09]
- EMBO Long-Term fellowship [ALTF 1128-2016]
- NIH F32 Postdoctoral Fellowship
- Burroughs Wellcome PDEP award
- Allen Discovery Center at Stanford University
- Crowdflower A.I. for everyone award
- NIH [5R01CA18390402, 1DP2OD022550-01, 1-R00-GM104148-01, 5U19AI116484-02, U19 AI104209, 1-DP5-OD019822, 1R01AG056287-01, 1R01AG057915-01, 1U24CA224309-01]
- Bill and Melinda Gates Foundation
- Translational Research Award from the Stanford Cancer Institute
- NSF [ECCS-1542152]
The immune system is critical in modulating cancer progression, but knowledge of immune composition, phenotype, and interactions with tumor is limited. We used multiplexed ion beam imaging by time-of-flight (MIBI-TOF) to simultaneously quantify in situ expression of 36 proteins covering identity, function, and immune regulation at sub-cellular resolution in 41 triple-negative breast cancer patients. Multi-step processing, including deep-learning-based segmentation, revealed variability in the composition of tumor-immune populations across individuals, reconciled by overall immune infiltration and enriched co-occurrence of immune subpopulations and checkpoint expression. Spatial enrichment analysis showed immune mixed and compartmentalized tumors, coinciding with expression of PD1, PD-L1, and IDO in a cell-type- and location-specific manner. Ordered immune structures along the tumor-immune border were associated with compartmentalization and linked to survival. These data demonstrate organization in the tumor-immune microenvironment that is structured in cellular composition, spatial arrangement, and regulatory-protein expression and provide a framework to apply multiplexed imaging to immune oncology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据