期刊
CELL
卷 159, 期 4, 页码 940-954出版社
CELL PRESS
DOI: 10.1016/j.cell.2014.10.004
关键词
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资金
- Canadian Institutes of Health Research
- Wyss Institute
- NIH Director's New Innovator Award [1DP2OD007292]
- ONR Young Investigator Program Award [N000141110914]
- NSF Expedition in Computing Award [CCF1317291]
- Howard Hughes Medical Institute
- Office of Naval Research MURI program
- Defense Threat Reduction Agency [HDTRA1-14-1-0006]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1317694] Funding Source: National Science Foundation
Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid proto-typing of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors.
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