期刊
CELL
卷 159, 期 4, 页码 911-924出版社
CELL PRESS
DOI: 10.1016/j.cell.2014.10.034
关键词
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资金
- Program for Innovative Cell Biology, Innovative Technology
- Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- MEXT/Japan Society for the Promotion of Science (JSPS) [25221004, 23115006, 26-1180]
- strategic programs for R&D (President's discretionary fund) of RIKEN
- RIKEN Center for Developmental Biology
- RIKEN Quantitative Biology Center
- Core Research for Evolutional Science and Technology (CREST)
- Japan Science and Technology Agency (JST)
- Japan Foundation for Applied Enzymology
- Shimabara Science Promotion Foundation
- RIKEN Foreign Postdoctoral Researcher Program
The development of whole-body imaging at single-cell resolution enables system-level approaches to studying cellular circuits in organisms. Previous clearing methods focused on homogenizing mismatched refractive indices of individual tissues, enabling reductions in opacity but falling short of achieving transparency. Here, we show that an aminoalcohol decolorizes blood by efficiently eluting the heme chromophore from hemoglobin. Direct transcardial perfusion of an aminoalcohol-containing cocktail that we previously termed CUBIC coupled with a 10 day to 2 week clearing protocol decolorized and rendered nearly transparent almost all organs of adult mice as well as the entire body of infant and adult mice. This CUBIC-perfusion protocol enables rapid whole-body and whole-organ imaging at single-cell resolution by using light-sheet fluorescent microscopy. The CUBIC protocol is also applicable to 3D pathology, anatomy, and immunohistochemistry of various organs. These results suggest that whole-body imaging of colorless tissues at high resolution will contribute to organism-level systems biology.
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