期刊
CELL
卷 157, 期 6, 页码 1460-1472出版社
CELL PRESS
DOI: 10.1016/j.cell.2014.04.028
关键词
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资金
- Wellcome Trust [093966/Z/10/Z, G1000236, WT090323MA, 084957/Z/08/Z]
- MRC
- National Institute of Health [GM067945]
- Cambridge Biomedical Research Centre, UK
- Medical Research Council [G1000236, MR/L008734/1, MR/L018373/1, G0901119] Funding Source: researchfish
- Wellcome Trust [090323/Z/09/Z] Funding Source: researchfish
- Wellcome Trust [093966/Z/10/Z, 084957/Z/08/Z, 090323/Z/09/Z] Funding Source: Wellcome Trust
- MRC [G0901119, MR/L008734/1, G1000236] Funding Source: UKRI
A systematic quantitative analysis of temporal changes in host and viral proteins throughout the course of a productive infection could provide dynamic insights into virus-host interaction. We developed a proteomic technique called quantitative temporal viromics (QTV), which employs multiplexed tandem-mass-tag-based mass spectrometry. Human cytomegalovirus (HCMV) is not only an important pathogen but a paradigm of viral immune evasion. QTV detailed how HCMV orchestrates the expression of >8,000 cellular proteins, including 1,200 cell-surface proteins to manipulate signaling pathways and counterintrinsic, innate, and adaptive immune defenses. QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets. Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined. QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.
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