期刊
ANALYTICAL SCIENCES
卷 19, 期 1, 页码 167-169出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.19.167
关键词
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A simple method to label oligosaccharides With a multifunctional fluorescent group was developed. Oligosaccharides were quantitatively labeled at their reducing termini with pyrene butanoic acid hydrazide. The pyrene-labeled oligosaccharides were successfully applied to fluorescence polarization measurements and ELISA at picomole quantity, which was not previously reached by other procedures. This labeling method should prove to be useful in a variety of aspects in glycobiology.
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