Microbulbifer salipaludis sp nov., a. moderate halophile isolated from a Korean salt marsh

A Gram-negative, non-motile, non-spore-forming, moderately halophilic rod (strain SM-1(T)) was isolated from salt marsh around the junction of the Youngsan River and the Yellow Sea in Korea and subjected to a polyphasic taxonomic study. This organism grew optimally at 37 degreesC and was able to grow at 10 and 45 degreesC. It grew optimally in the presence of 2-3 % (w/v) NaCl. The major fatty acids in strain SM-1(T) were iso-C-15:0 and C-16:0. Strain SM-1(T) and Microbulbifer hydrolyticus DSM 11525 T were characterized by having ubiquinone-8 as the predominant respiratory lipoquinone. The DNA G+C content of strain SM-1(T) was 59 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain SM-1(T) formed a coherent cluster with M. hydrolyticus; this relationship was supported by a bootstrap resampling value of 100 %. The level of 16S rDNA identity between strain SM-1(T) and the type strain of M. hydrolyticus was 98-6 %. The mean level of DNA-DNA relatedness between strain SM-1(T) and the type strain of M. hydrolyticus was 20.6 %. Therefore, on the basis of phenotypic properties, phylogeny and genomic data, strain SM-1(T) should be placed in the genus Microbulbifer as a member of a novel species, for which the name Microbulbifer salipaludis sp. nov. is proposed. The type strain of the novel species is strain SM-1(T) (=KCCM 41586(T) =JCM 11542(T)).