期刊
NATURE CELL BIOLOGY
卷 5, 期 11, 页码 987-993出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1055
关键词
-
类别
资金
- NATIONAL CANCER INSTITUTE [R01CA058541, T32CA009197] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R29NS035532] Funding Source: NIH RePORTER
- NCI NIH HHS [R01 CA058541, T32 CA009197] Funding Source: Medline
- NINDS NIH HHS [R29 NS35532] Funding Source: Medline
During early embryogenesis in Drosophila melanogaster, extensive vesicle transport occurs to build cell boundaries for 6,000 nuclei. Here we show that this important process depends on a functional complex formed between the tumour suppressor and adaptor protein Discs-Large (Dlg)(1) and the integral membrane protein Strabismus (Stbm)/Van Gogh (Vang)(2,3). In support of this idea, embryos with mutations in either dlg or stbm displayed severe defects in plasma membrane formation. Conversely, overexpression of Dlg and Stbm synergistically induced excessive plasma membrane formation. In addition, ectopic co-expression of Stbm (which associated with post-Golgi vesicles) and the mammalian Dlg homologue SAP97/hDlg(4,5) promoted translocation of SAP97 from the cytoplasm to both post-Golgi vesicles and the plasma membrane. This effect was dependent on the interaction between Stbm and SAP97. These findings suggest that the Dlg-Stbm complex recruits membrane-associated proteins and lipids from internal membranes to sites of new plasma membrane formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据