期刊
CELL
卷 150, 期 1, 页码 222-232出版社
CELL PRESS
DOI: 10.1016/j.cell.2012.05.033
关键词
-
资金
- NSF [0821391]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0844442] Funding Source: National Science Foundation
Orthologous proteins often harbor numerous substitutions, but whether these differences result from neutral or adaptive processes is usually unclear. To tackle this challenge, we examined the divergent evolution of a model bacterial signaling pathway comprising the kinase PhoR and its cognate substrate PhoB. We show that the specificity-determining residues of these proteins are typically under purifying selection but have, in alpha-proteobacteria, undergone a burst of diversification followed by extended stasis. By reversing mutations that accumulated in an alpha-proteobacterial PhoR, we demonstrate that these substitutions were adaptive, enabling PhoR to avoid crosstalk with a paralogous pathway that arose specifically in alpha-proteobacteria. Our findings demonstrate that duplication and the subsequent need to avoid crosstalk strongly influence signaling protein evolution. These results provide a concrete example of how system-wide insulation can be achieved postduplication through a surprisingly limited number of mutations. Our work may help explain the apparent ease with which paralogous protein families expanded in all organisms.
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