期刊
ELECTROANALYSIS
卷 15, 期 23-24, 页码 1815-1823出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200302764
关键词
graphite-epoxy composite; DNA; hybridization; enzyme labeling; nylon membrane; amperometric genosensors; DNA biosensor; Salmonella spp; IS200
A PCR-genosensor based assay for detecting the IS200 DNA sequence specific to Salmonella spp is reported. This rapid test is based on PCR, where amplicon detection is achieved electrochemically using GEC (graphite-epoxy composite) genosensor. The amplicon is immobilized onto GEC electrodes by simple dry-adsorption and the detection is carried out using an enzymatic labeling system. Results demonstrate that this new electrochemical genosensor fulfils the requirements desired for these devices: easy preparation - as dry-adsorption of DNA is very simple -, robustness, sensitivity, low cost, simple use and fast response. Additionally, the assay can be produced as a kit format. increasing its commercial potentiality. Also, the electrode material can be implemented for screen-printing procedures for the mass production of genosensors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据