期刊
CELL
卷 151, 期 4, 页码 765-777出版社
CELL PRESS
DOI: 10.1016/j.cell.2012.10.019
关键词
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资金
- Research Center Program of IBS (Institute for Basic Science) [EM1202]
- BK21 Research Fellowships from the Ministry of Education, Science and Technology of Korea
- TJ Park Postdoctoral Fellowship
- National Honor Scientist Program through the National Research Foundation of Korea (NRF) [20100020415]
- Ministry of Education, Science & Technology (MoST), Republic of Korea [IBS EM1202] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [과06A1204, 2010-0020415] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
LIN28 plays a critical role in developmental transition, glucose metabolism, and tumorigenesis. At the molecular level, LIN28 is known to repress maturation of let-7 microRNAs and enhance translation of certain mRNAs. In this study, we obtain a genome-wide view of the molecular function of LIN28A in mouse embryonic stem cells by carrying out RNA crosslinking-immunoprecipitation-sequencing (CLIP-seq) and ribosome footprinting. We find that, in addition to let-7 precursors, LIN28A binds to a large number of spliced mRNAs. LIN28A recognizes AAGNNG, AAGNG, and less frequently UGUG, which are located in the terminal loop of a small hairpin. LIN28A is localized to the periendo-plasmic reticulum (ER) area and inhibits translation of mRNAs that are destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Our study suggests a selective regulatory mechanism for ER-associated translation and reveals an unexpected role of LIN28A as a global suppressor of genes in the secretory pathway.
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