期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 283, 期 1-2, 页码 17-25出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2003.07.003
关键词
HIV; antibody; phage display; gp120; inhibitors; vaccines
资金
- NCI NIH HHS [N01-CO-12400] Funding Source: Medline
Identification of broadly cross-reactive human monoclonal antibodies (mAbs) has major implications for development of vaccines, inhibitors and research tools. Here we describe a sequential antigen panning (SAP) methodology that may facilitate the selection of such antibodies. An HIV-specific antibody Fab (m18) was selected from a human Fab phage-display library by SAP against several recombinant soluble HIV envelope glycoproteins (Envs) and Env-sCD4 complexes. This Fab bound to a variety of recombinant soluble Envs (gp140s) from primary HIV isolates representing different clades, and inhibited cell fusion and virus entry mediated by Envs of primary HIV isolates. The methodology and the results may have implications for development of HIV vaccines and inhibitors, as well as for identification of antibodies to conserved epitopes on rapidly mutating viruses and cells. Published by Elsevier B.V.
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