期刊
MOLECULAR ECOLOGY NOTES
卷 3, 期 4, 页码 483-486出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-8286.2003.00485.x
关键词
grasshopper; Locusta migratoria manilensis; migration; Orthoptera; repetitive microsatellite locus
Eight polymorphic di- and trinucleotide microsatellite loci suitable for population genetic analysis were developed in Locusta migratoria from a partial phagemid genomic library enriched for microsatellite inserts. The expected heterozygosity at these loci ranges from 0.45 to 0.97, with the observed allele numbers varying between nine and 45. The overall microsatellite cloning efficiency in L. migratoria is 14%, suggesting that in migratory locusts, microsatellite sequences are abundant and should provide a valuable and easily accessible source of nuclear markers for genetic studies. These microsatellite loci were highly Locusta-specific, with only very limited cross-species applicability.
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