期刊
CELL
卷 145, 期 1, 页码 117-132出版社
CELL PRESS
DOI: 10.1016/j.cell.2011.02.039
关键词
-
资金
- Marie Curie Excellence grant [MEXT-CT-2006-042267]
- ERC [260678]
- FWO [G074709, G094011]
- Research Fund
- Flemish Government
- Francqui Foundation
- VIB
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
- European Research Council (ERC) [260678] Funding Source: European Research Council (ERC)
Exchange of proteins at sorting endosomes is not only critical to numerous signaling pathways but also to receptor-mediated signaling and to pathogen entry into cells; however, how this process is regulated in synaptic vesicle cycling remains unexplored. In this work, we present evidence that loss of function of a single neuronally expressed GTPase activating protein (GAP), Skywalker (Sky) facilitates endosomal trafficking of synaptic vesicles at Drosophila neuromuscular junction boutons, chiefly by controlling Rab35 GTPase activity. Analyses of genetic interactions with the ESCRT machinery as well as chimeric ubiquitinated synaptic vesicle proteins indicate that endosomal trafficking facilitates the replacement of dysfunctional synaptic vesicle components. Consequently, sky mutants harbor a larger readily releasable pool of synaptic vesicles and show a dramatic increase in basal neurotransmitter release. Thus, the trafficking of vesicles via endosomes uncovered using sky mutants provides an elegant mechanism by which neurons may regulate synaptic vesicle rejuvenation and neurotransmitter release.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据