期刊
CELL
卷 146, 期 4, 页码 645-658出版社
CELL PRESS
DOI: 10.1016/j.cell.2011.06.051
关键词
-
资金
- Australian Research Council
- National Health and Medical Research Council of Australia
- Queensland Government Department of Employment, Economic Development and Innovation
- Australian Stem Cell Centre
- NIH [U54HG004592, U54HG004592-S1]
The human mitochondrial genome comprises a distinct genetic system transcribed as precursor poly-cistronic transcripts that are subsequently cleaved to generate individual mRNAs, tRNAs, and rRNAs. Here, we provide a comprehensive analysis of the human mitochondrial transcriptome across multiple cell lines and tissues. Using directional deep sequencing and parallel analysis of RNA ends, we demonstrate wide variation in mitochondrial transcript abundance and precisely resolve transcript processing and maturation events. We identify previously undescribed transcripts, including small RNAs, and observe the enrichment of several nuclear RNAs in mitochondria. Using high-throughput in vivo DNaseI footprinting, we establish the global profile of DNA-binding protein occupancy across the mitochondrial genome at single-nucleotide resolution, revealing regulatory features at mitochondrial transcription initiation sites and functional insights into disease-associated variants. This integrated analysis of the mitochondrial transcriptome reveals unexpected complexity in the regulation, expression, and processing of mitochondrial RNA and provides a resource for future studies of mitochondrial function (accessed at http://mitochondria.matticklab.com).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据