期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 67, 期 2, 页码 503-514出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erv416
关键词
Arabidopsis; Brachypodium; cellulose microfibrils; dynamics; intermolecular contact; multidimensional correlation NMR
资金
- Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center - US Department of Energy, Office of Science, Basic Energy Sciences [DE-SC0001090]
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [P41EB002026] Funding Source: NIH RePORTER
This article discusses the use of multidimensional solid-state NMR to site-specifically detect polysaccharide interactions and the chain numbers of cellulose microfibrils in near-native primary cell wall samples.Until recently, the 3D architecture of plant cell walls was poorly understood due to the lack of high-resolution techniques for characterizing the molecular structure, dynamics, and intermolecular interactions of the wall polysaccharides in these insoluble biomolecular mixtures. We introduced multidimensional solid-state NMR (SSNMR) spectroscopy, coupled with C-13 labelling of whole plants, to determine the spatial arrangements of macromolecules in near-native plant cell walls. Here we review key evidence from 2D and 3D correlation NMR spectra that show relatively few cellulose-hemicellulose cross peaks but many cellulose-pectin cross peaks, indicating that cellulose microfibrils are not extensively coated by hemicellulose and all three major polysaccharides exist in a single network rather than two separate networks as previously proposed. The number of glucan chains in the primary-wall cellulose microfibrils has been under active debate recently. We show detailed analysis of quantitative C-13 SSNMR spectra of cellulose in various wild-type (WT) and mutant Arabidopsis and Brachypodium primary cell walls, which consistently indicate that primary-wall cellulose microfibrils contain at least 24 glucan chains.
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