期刊
CELL
卷 142, 期 3, 页码 398-408出版社
CELL PRESS
DOI: 10.1016/j.cell.2010.06.034
关键词
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资金
- Centre National de Recherche Scientifique (France)
- ANR
- European contract [LSHM-CT-2005-019023]
- Commissariat a l'Energie Atomique and Electricite de France (France)
- National Institute of Diabetes and Digestive and Kidney Diseases
DNA transposition has contributed significantly to evolution of eukaryotes and prokaryotes. Insertion sequences (ISs) are the simplest prokaryotic trans-posons and are divided into families on the basis of their organization and transposition mechanism. Here, we describe a link between transposition of IS608 and ISDra2, both members of the IS200/IS605 family, which uses obligatory single-stranded DNA intermediates, and the host replication fork. Replication direction through the IS plays a crucial role in excision: activity is maximal when the top'' IS strand is located on the lagging-strand template. Excision is stimulated upon transient inactivation of replicative helicase function or inhibition of Okazaki fragment synthesis. IS608 insertions also exhibit an orientation preference for the lagging-strand template and insertion can be specifically directed to stalled replication forks. An in silico genomic approach provides evidence that dissemination of other IS200/IS605 family members is also linked to host replication.
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