Novel fluorescent Mg2+ probes, 2(')-carboxyfluorescein (2(')-CF) and its derivatives, that are excitable by visible light, were designed, synthesized and characterized. After complexation with Mg2+, the absorption and emission maxima of these fluorescent probes were red-shifted and the fluorescence intensity was increased 11-fold at neutral pH. The apparent dissociation constant (K-d) of the Mg2+-2'-CF complex was 15.8 mM at neutral pH. The K-d of the Ca2+-2'-CF complex was about 10 times larger than that of the Mg2+ complex and the other derivatives showed similar characteristics. In contrast, all the commercially available fluorescent Mg2+ probes have a higher affinity for Ca2+ than Mg2+. 2'-CF fluoresced in alkaline solution (pH > 8) and the pK(a) value was 8.8. The pK(a) value of the Mg2+-2'-CF complex was 6.8, and the fluorescence intensity was increased in the neutral conditions. Thus, the addition of Mg2+ resulted in a lowering of the pK(a) and also an increase of the fluorescence intensity.
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