期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 27, 期 1, 页码 27-34出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1046-5928(02)00574-0
关键词
AcMNPV; baculovirus; larvae; hepatitis E virus; ORF2
We report here the expression and purification of a truncated form of the hepatitis E virus ORF2 protein (ORF2Delta111/DeltaTM), from the fat bodies of Spodoptera litura larvae infected with a recombinant baculovirus. The purified protein migrated as a doublet of similar to56 kDa on SDS-PAGE and was found to be glycosylated by staining with concanavalin A-linked horseradish peroxidase. The protein was used in a sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to HEV. The results showed complete concordance with those obtained using a commercial kit for the detection of anti-HEV antibodies. Antigen expression in the insect larvae system presents a rapid and low-cost method that obviates the need for expensive tissue culture scale-ups or special equipment. (C) 2002 Elsevier Science (USA). All rights reserved.
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