期刊
CELL
卷 138, 期 4, 页码 722-737出版社
CELL PRESS
DOI: 10.1016/j.cell.2009.07.039
关键词
-
资金
- The Wellcome Trust
- Biotechnology and Biological Sciences Research Council
- Medical Research Council
- EC Framework 7
- Medical Research Council [G0300723B, G0700711B, G0700665, G9806702] Funding Source: researchfish
- MRC [G0700665, G9806702] Funding Source: UKRI
Pluripotency is generated naturally during mammalian development through formation of the epiblast, founder tissue of the embryo proper. Pluripotency can be recreated by somatic cell reprogramming. Here we present evidence that the homeodomain protein Nanog mediates acquisition of both embryonic and induced pluripotency. Production of pluripotent hybrids by cell fusion is promoted by and dependent on Nanog. In transcription factor-induced molecular reprogramming, Nanog is initially dispensable but becomes essential for dedifferentiated intermediates to transit to ground state pluripotency. In the embryo, Nanog specifically demarcates the nascent epiblast, coincident with the domain of X chromosome reprogramming. Without Nanog, pluripotency does not develop, and the inner cell mass is trapped in a pre-pluripotent, indeterminate state that is ultimately nonviable. These findings suggest that Nanog choreographs synthesis of the naive epiblast ground state in the embryo and that this function is recapitulated in the culmination of somatic cell reprogramming.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据