期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 66, 期 18, 页码 5567-5580出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erv176
关键词
Arabidopsis thaliana; genome-wide association mapping; growth dynamics; GWAS; natural variation; PLA; plant phenotyping; projected leaf area; rosette growth
资金
- Dutch Technology Foundation (STW) of the Netherlands Organisation for Scientific Research (NWO) [1099]
- European Plant Phenotyping Network (EPPN) by European Union [284443]
Growth curve modelling and GWA mapping are combined to unravel the dynamic regulation of plant growth.Growth is a complex trait determined by the interplay between many genes, some of which play a role at a specific moment during development whereas others play a more general role. To identify the genetic basis of growth, natural variation in Arabidopsis rosette growth was followed in 324 accessions by a combination of top-view imaging, high-throughput image analysis, modelling of growth dynamics, and end-point fresh weight determination. Genome-wide association (GWA) mapping of the temporal growth data resulted in the detection of time-specific quantitative trait loci (QTLs), whereas mapping of model parameters resulted in another set of QTLs related to the whole growth curve. The positive correlation between projected leaf area (PLA) at different time points during the course of the experiment suggested the existence of general growth factors with a function in multiple developmental stages or with prolonged downstream effects. Many QTLs could not be identified when growth was evaluated only at a single time point. Eleven candidate genes were identified, which were annotated to be involved in the determination of cell number and size, seed germination, embryo development, developmental phase transition, or senescence. For eight of these, a mutant or overexpression phenotype related to growth has been reported, supporting the identification of true positives. In addition, the detection of QTLs without obvious candidate genes implies the annotation of novel functions for underlying genes.
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