期刊
CELL
卷 139, 期 1, 页码 123-134出版社
CELL PRESS
DOI: 10.1016/j.cell.2009.09.014
关键词
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资金
- Fundacao para Ciencia e a Tecnologia, Portugal [SFRH/BD/11803/2003, SFRH/BD/17629/2004/H6BM]
- NIGMS [GMO58800]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/17629/2004, SFRH/BD/11803/2003] Funding Source: FCT
RNAi-related pathways regulate diverse processes, from developmental timing to transposon silencing. Here, we show that in C. elegans the Argonaute CSR-1, the RNA-dependent RNA polymerase EGO-1, the Dicer-related helicase DRH-3, and the Tudordomain protein EKL-1 localize to chromosomes and are required for proper chromosome segregation. In the absence of these factors chromosomes fail to align at the metaphase plate and kinetochores do not orient to opposing spindle poles. Surprisingly, the CSR-1-interacting small RNAs (22G-RNAs) are antisense to thousands of germline-expressed protein-coding genes. Nematodes assemble holocentric chromosomes in which continuous kinetochores must span the expressed domains of the genome. We show that CSR-1 interacts with chromatin at target loci but does not downregulate target mRNA or protein levels. Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans.
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