Mapping of chlorina mutant genes on the long arm of homoeologous group 7 chromosomes in common wheat with partial deletion lines

The chlorophyll a:b ratio of chlorina mutants is much higher than that of wild type plants. Physical mapping of the chlorina mutant loci (cn-A1, cn-B1 and cn-D1) of common wheat (Triticum aestivum L.) and durum wheat (T turgidum L.) was carried out with partial deletion lines of Chinese Spring (CS) of the long arms of homoeologous group 7 chromosomes. F, plants of partial deletion lines with near-isogenic lines (ANK-32A and ANK-32B) of the spring bread wheat Novosibirskaya 67 and a near-isogenic line of durum wheat LD222, ANW-7B were evaluated for chlorophyll a:b ratio of the leaves. Hemizygous and heterozygous plants were more easily distinguished by chlorophyll a:b ratio than by visual observation. The dose effects of the chlorina loci on chlorophyll a:b ratio were also confirmed. The position of the allele on the chromosome was localized by fraction length, the comparative values between whole chromosome and partially deleted chromosome. The locus cn-A1 was localized on the region of 83% distal from the centromere on the long arm of chromosome 7A, cn-B1 locus was localized on the region between 69% and 78% distal from the centromere on the long arm of chromosome 7B, and cn-D1 locus was localized on the region between 76% and 77% distal from the centromere on the long arm of chromosome 7D. We consider the map derived by deletion mapping is more accurate than the map calculated from recombination frequency.