期刊
CELL
卷 137, 期 3, 页码 485-497出版社
CELL PRESS
DOI: 10.1016/j.cell.2009.02.040
关键词
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资金
- ANR [NT05-4_42267, PCV06_142302]
- Ligue Nationale contre le Cancer (Equipe labellise'e la Ligue)
- PIC Programs
- European Commission Network of Excellence Epigenome [LSHG-CT-2004-503433]
- Canceropole IdF Breast cancer and Epigenetics'' [ACI-2007]
The histone H3 variant CenH3, called CENP-A in humans, is central in centromeric chromatin to ensure proper chromosome segregation. In the absence of an underlying DNA sequence, it is still unclear how CENP-A deposition at centromeres is determined. Here, we purified non-nucleosomal CENP-A complexes to identify direct CENP-A partners involved in such a mechanism and identified HJURP. HJURP was not detected in H3.1- or H3.3-containing complexes, indicating its specificity for CENP- A. HJURP centromeric localization is cell cycle regulated, and its transient appearance at the centromere coincides precisely with the proposed time window for new CENP- A deposition. Furthermore, HJURP downregulation leads to a major reduction in CENP-A at centromeres and impairs deposition of newly synthesized CENP-A, causing mitotic defects. We conclude that HJURP is a key factor for CENP-A deposition and maintenance at centromeres.
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