期刊
BIOCHEMISTRY-MOSCOW
卷 68, 期 11, 页码 1247-1251出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1023/B:BIRY.0000009140.50032.3d
关键词
anion channel; anion co-transporter; NPPB; DIDS; furosemide; bumetanide; DiBAC(4)(5); pollen germination
The role of Cl- transport across the plasma membrane was studied in an early step of pollen grain germination in tobacco Nicotiana tabacum L. The Cl- channel blockers, 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and niflumic acid, completely suppress the germination with IC50 similar to8 muM. At this concentration NPPB reduces the rate of Cl- efflux out of pollen grain by 1.8-fold in the interval 5-12 min, and niflumic acid reduces the rate 1.2-fold. 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid, a known inhibitor of Cl- channels and antiporters, completely suppresses germination as well (IC50=240 muM), but has no effect on the rate of Cl- efflux. Inhibitors of chloride co-transporters, such as furosemide, bumetanide, and bis(1,3-dibutylbarbituric acid)pentamethine oxonol, suppress the germination by less than 50%. This set of data suggests that NPPB-sensitive anion channels are involved in the activation of pollen grains in the early stage of germination.
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