期刊
CELL
卷 134, 期 4, 页码 599-609出版社
CELL PRESS
DOI: 10.1016/j.cell.2008.06.033
关键词
-
资金
- National Institutes of Health [GM45744, GM67825, HG003079]
The Drosophila MSL complex associates with active genes specifically on the male X chromosome to acetylate histone H4 at lysine 16 and increase expression approximately 2-fold. To date, no DNA sequence has been discovered to explain the specificity of MSL binding. We hypothesized that sequence-specific targeting occurs at chromatin entry sites,'' but the majority of sites are sequence independent. Here we characterize 150 potential entry sites by ChIP-chip and ChIP-seq and discover a GA-rich MSL recognition element (MRE). The motif is only slightly enriched on the X chromosome (similar to 2fold), but this is doubled when considering its preferential location within or 30 to active genes (> 4-fold enrichment). When inserted on an autosome, a newly identified site can direct local MSL spreading to flanking active genes. These results provide strong evidence for both sequence-dependent and -independent steps in MSL targeting of dosage compensation to the male X chromosome.
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