期刊
CELL CYCLE
卷 2, 期 5, 页码 479-483出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.2.5.483
关键词
Rad52; foci; homologous recombination; checkpoint; Saccharomyces cerevisiae
类别
资金
- Danish Natural Science Research Council
- Danish Technical Research Council
- Alfred Benzon Foundation
- NIH [GM50237, GM67055]
- Tonnessen Foundation
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM050237, R01GM067055, R37GM050237] Funding Source: NIH RePORTER
In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA doublestrand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.
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