期刊
PLANT CELL REPORTS
卷 21, 期 5, 页码 408-414出版社
SPRINGER-VERLAG
DOI: 10.1007/s00299-002-0544-y
关键词
Saintpaulia ionantha Wendl.; morphogenesis; regeneration; auxin; cytokinin; hidiazuron
Regeneration via shoot organogenesis and somatic embryogenesis was observed from thidiazuron (TDZ)-treated leaf and petiole explants of greenhouse and in vitro-grown African violet plants. The response of cultures to other growth regulators over a range of 0.5 muM to 10 muM was 50% less than that observed with TDZ. A comparative study among several cultivars of African violet indicated that 'Benjamin' and 'William' had the highest regeneration potential. In 'Benjamin', higher frequencies of shoot organogenesis (twofold) and somatic embryogenesis (a 50% increase) were observed from in vitro- and greenhouse-grown plants, respectively. At concentrations lower than 2.5 muM, TDZ induced shoot organogenesis, whereas at higher doses (5-10 muM) somatic embryos were formed. These findings provide the first report of simultaneous shoot organogenesis and somatic embryogenesis of African violet explants in response to TDZ.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据