期刊
RNA
卷 9, 期 1, 页码 9-13出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.2990203
关键词
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资金
- NIGMS NIH HHS [R01 GM016317, F32 GM016317, GM16317] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [F32GM016317, R01GM016317] Funding Source: NIH RePORTER
It has been reported that nuclei from HeLa cells are responsible for similar to10%-15% of total cellular protein synthesis. We show here that isolated Chinese hamster ovary (CHO) and HeLa cell nuclei are essentially inactive for translation, and that the earlier results were most likely due to cytoplasmic contamination. Moreover, we suggest that the nascent polypeptides observed in nuclei of permeabilized cells may have been due to overpermeabilization and consequent damage to the cells. Based on this information, we conclude that nuclear protein synthesis, if it exists, is limited to less than 1% of that in cells.
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