Bacteriocin-like substance production by Bacillus licheniformis strain P40

Aims: To investigate the production of bacteriocin-like compounds by Bacillus spp. isolated from the Amazon basin. Methods and Results: An antimicrobial substance produced by Bacillus licheniformis strain P40 was inhibitory to a broad range of indicator strains, such as Listeria monocytogenes, Bacillus cereus and clinical isolates of Streptococcus spp. The compound was stable at 100degreesC, but lost its activity when treated at 121degreesC/103.5 kPa for 15 min. It was resistant to the proteolytic action of trypsin and papain but sensitive to pronase E and was stable within a wide range of pH (3-11). The substance was bactericidal and bacteriolytic to L. monocytogenes. Conclusions: An antibacterial peptide produced by Bacillus licheniformis was characterized, presenting a broad spectrum of activity against pathogenic and spoilage organisms. Significance and Impact of the Study: The identification of a substance active against important pathogens addresses an important aspect of food safety.