期刊
CELL BIOCHEMISTRY AND BIOPHYSICS
卷 41, 期 3, 页码 357-365出版社
HUMANA PRESS INC
DOI: 10.1385/CBB:41:3:357
关键词
formate hydrogenlyase; pH; Escherichia coli
Fermenting Escherichia coli is able to produce formate and molecular hydrogen (H-2) when grown on glucose. H-2 formation is possessed by two hydrogenases, 3 (Hyd-3) and 4 (Hyd-4), those, in conjunction with formate dehydrogenase H (Fdh-H), constitute distinct membrane-associated formate hydrogenylases. At slightly alkaline pH (pH 7.5), the production of H2 was found to be dependent on Hyd-4 and the F0F1-adenosine triphosphate (ATPase), whereas external formate increased the activity of Hyd-3. In this study with cells grown without and with external formate, H-2 production dependent on pH was investigated. In both types of cells, H2 production was increased after lowering of pH. At acidic pH (pH 5.5), this production became insensitive either to N,N'-dicyclohexylcarbodiimide or to osmotic shock and it became largely dependent on Fdh-H and Hyd-3 but not Hyd-4 and the F0F1-ATPase. The results indicate that Hyd-3 has a major role in H-2 production at acidic pH independently on the F0F1-ATPase.
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