期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 34, 期 1, 页码 109-115出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2003.10.002
关键词
Haemonchus contortus; nematode; anthelmintics; drug resistance; amplified fragment length polymorphism; inbreeding
类别
Haemonchus contortus is a sheep parasitic nematode that causes severe economic losses. Previous studies have indicated a high degree of genetic heterogeneity, which is hardly affected by selection for drug resistance. As a tool for the analysis of the population dynamics of H. contortus and its response to drug resistance, we designed a strategy to study the inbreeding of a benzimidazole-sensitive and a benzimidazole-resistant strain. After 15 generations, a theoretical inbreeding coefficient of 0.87 was achieved. The different stages of inbreeding were analysed using restriction fragment polymorphism, microsatellite variability and amplified fragment length polymorphism. Model-based clustering of the amplified fragment length polymorphism genotypes showed that the allele frequencies of the benzimidazole-resistant strain were stable during the last eight generations. In the sensitive strain a gradual shift of allele frequencies was observed, which led to a temporary increase of the genetic diversity around the eight generations. (C) 2004 on behalf of Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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