期刊
MICRON
卷 35, 期 4, 页码 229-234出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micron.2003.11.015
关键词
clara cell; nissl stain; real-time polymerase chain reaction; microarray
类别
资金
- NHLBI NIH HHS [HL 47328, HL 29594] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL047328, P01HL029594] Funding Source: NIH RePORTER
The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells. (C) 2003 Elsevier Ltd. All rights reserved.
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