期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 63, 期 1, 页码 18-22出版社
WILEY
DOI: 10.1002/jemt.10423
关键词
confocal; second harmonic; multiphoton; resolution; super-resolution
Calculated and measured resolution figures are presented for confocal microscopes with different pinhole sizes and for nonlinear (2-photon and second harmonic) microscopes. A modest degree of super-resolution is predicted for a confocal microscope but in practice this is not achievable and confocal fluorescence gives little resolution improvement over widefield. However, practical non-linear microscopes do approach their theoretical resolution and therefore show no resolution disadvantage relative to confocal microscopes in spite of the longer excitation wavelength.
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