期刊
CELLULAR SIGNALLING
卷 16, 期 1, 页码 43-49出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0898-6568(03)00096-2
关键词
RGS; heterologous expression; G-protein coupled receptors; yeast; growth inhibition
类别
Regulators of G-protein Signalling (RGS) regulate the functional lifetime of G-Protein Coupled Receptor (GPCR)-activated heterotrimeric G-protein by serving as GTPase Accelerating Proteins (GAPs) for the G. subunit. A number of mammalian RGSs can functionally replace the yeast RGS containing SST2 gene and inhibit GPCR signalling. Using yeast strains harbouring a G(betagamma)-responsive FUS1-LacZ reporter gene, we demonstrate that heterologously expressed mammalian RGS I also serves to decrease basal signalling in the absence of agonist. Although this effect was dependent on the expression of a GPA1-encoded functional G, protein, the GPCR itself was nevertheless not required. Using the GAL1 inducible promoter to express RGS 1, we further demonstrate that in addition to serving as a GAP for Gpa1p in yeast, RGS1 is a dosage-dependent inhibitor of growth. This effect is specific to RGS1 since growth is not altered in cells expressing either mammalian RGS2 or RGS5. We further demonstrate that neither of the two yeast G. proteins is responsible for mediating this growth inhibitory effect of RGS1. Taken together, our results indicate that RGS I can function in both G-protein-dependent and -independent manners in yeast. (C) 2003 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据