期刊
MICROBIOLOGY AND IMMUNOLOGY
卷 48, 期 5, 页码 389-398出版社
WILEY
DOI: 10.1111/j.1348-0421.2004.tb03528.x
关键词
aerobactin operon; ABC transport system; iron uptake; iron-regulated gene
In response to iron deprivation, Vibrio mimicus produces aerobactin as a major siderophore. Application of the Fur titration assay to a V mimicus genomic DNA library followed by further cloning of the surrounding regions led to the identification of two adjacent, iron-regulated operons. One contains three genes encoding homologs of the Escherichia coli FhuCDB and the other, five genes encoding homologs of the E. coli IucABCD IutA. Construction of the V mimicus polar disruptants in the respective operons allowed us to confirm their functions. The genetic arrangement of the aerobactin-mediated iron acquisition system in V mimicus is unique in that the aerobactin operon (iucABCD iutA) is contiguous to the operon (matCDB) encoding components of an ATP-binding cassette transport system for ferric aerobactin. This is the first report demonstrating that aerobactin transport and biosynthesis genes are present in a species outside the family Enterobacteriaceae.
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